Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTVPQNMKAAVMHNTRE-IKIETLPVPDINHDEVLIKVMAVGICGSDLHYYTNGRIGNYVVEK--PFILGHECAGEIAAVGSSVDQFKVGDRVAVEPGV--TCGRCEACKEGRYNLCPDVQFLATPPVDGAFVQYIKMRQDFVFLIPDSLSYEEAALIEPFSVGIHAAAR-TKLQPGSTIAIMGMGPVGLMAVAAAKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQDALEEIKTITNDRGVDVAWETAGNPAALQSALASVRRGGKLAIVGLPSQNEIPLNVPFIADNEIDIYG-IFRYANTYPKGIEFLASGIVDTKHLVTDQYSLEQTQDAMERALQFKNECLKVMVYPNR 3PII Chain:A ((1-325)) --------MKAAVVEQFKEPLKIKEVEKPTISYGEVLVRIKACGVCHTDLH----AAHGDWPVKPKLPLIPGHEGVGIVEEVGPGVTHLKVGDRVGI-PWLYSACGHCDYCLSGQETLC-EHQKNAGYSVDGGYAEYCRAAADYVVKIPDNLSFEEAAPIFCAGVTTYKALKVTGAKPGEWVAIYGIGGFGHVAVQYAKAMGL-NVVAVDIGDEKLELAKELGADLVVNPLKEDAAKFMKEKVG--GVHAAVVTAVSKPAFQSAYNSIRRGGACVLVGLPPE-EMPIPIFDTVLNGIKIIGSIVGTRKDLQEALQFAAEGKVKT---IIEVQPLEKINEVFDRMLK--------------

General information: TITO was launched using: RESULT: Template: 3PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1866 -248880 -133.38 -782.64 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -133.38 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_3PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PII-query.scw PDB file : Tito_Scwrl_3PII.pdb: