Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------MADVLRRAINQKKQFLKTKL--LLSEFYQGRGEQLADYTLSELEKEYKSLQKMKKEI-------------- 4FFB Chain:C ((11-272)) TLPLEERLTYKLWKARLEAYKELNQLFRNSQIYWRDPTLFAQYITDSNVVAQEQAIVALNSLIDAFALKNAHNITLISTWTPLLVEKGLTSSRATTKTQSMSCILSLCGLDTSITQSVELVIPFFEKKLPKLIAAAANCVYELMAAFGLTNVNVQTFLPELLKHVPQLAGHGDRNVRSQTMNLIVEIYKVLFKKLKPIQVKDLHKLFAKVGDLFEWEKRELEKKRSQEEEA

General information: TITO was launched using: RESULT: Template: 4FFB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 96 -18007 -187.57 -327.40 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.54 3D Compatibility (PKB) : -187.57 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_4FFB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFB-query.scw PDB file : Tito_Scwrl_4FFB.pdb: