Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MMEVGILPVHVLYVF-FIG--------AIILFMLLRKDTTFISLFGIFIISLWASHSLSASVSSLFHSFIYAAGELLPTIFIICFIVSMSDLLTKTGINEAMISPFASLVRGPVTAYWLIGGLMFAISLFFWPSPGVALIGA----------------------------------VLLPAAARAGL-----------------TPLAAAMAMN----LFGHGFALSGDFVIQAAPKLTADAAGIPVGDVV-SASIPLVLIMGVTTTTAAFIMIQR-----ERKKQEHPTSFSPVLS--GEQDNSLYLPKRLRSILAFLI-------PLAFLADIACML--LFNLQ----------GNDATALIGGSAICILFTVHFFVYKHKGLE---------KITGYFIDGFKFGF--KVFGPVIPIAAFFYLGDSGYESILG---TSLPKGSHGIVNDLGIALSHMMPM---SKELAATALTAAGAITGLDGSGFSGIS--LAGSIAKLFSSALHA----------DPAILTALGQISAI---------WVGGGTLVPWALIPAAAICKVDPFELARKNFIPVAIGLLVTTLAAVMML------------------------------------------------------------ 3TTC Chain:A ((12-657)) QIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPL---CPACDKEYRDPLDRRFHAQPVACPECGPY-------LEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDAR--NSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVVHDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGENGALWG-----GECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVAAANWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGE

General information: TITO was launched using: RESULT: Template: 3TTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2044 -271461 -132.81 -608.66 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -132.81 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_3TTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TTC-query.scw PDB file : Tito_Scwrl_3TTC.pdb: