Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALKAQNTISGKEGRLFLDGE-EMAHIKTFEANVEKNKSEVNIMGRRMTGHKTTGANGTGTATFYKVTSKFVLLMMDYVKKGSD-PYFTLQAVLDDQSSGRGTERVTLYDVNFDSAKIASLDVDSEALEEEVPFTFEDFDVPEKLSDTF 3EAA Chain:A ((11-133)) ----------KVDGESTDDKHKKWIEVLGFAWGAGNECTME-----SGTQGLNTGKAMMSVLRVTKWMDCASVKLASAAVQGQNFPTLELEICTQA-GDKFAFCIYKFTHVAVSSYQCSGATGGSDRPQETIDFAYKEV----------

General information: TITO was launched using: RESULT: Template: 3EAA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 430 -27969 -65.04 -231.14 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -65.04 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_3EAA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EAA-query.scw PDB file : Tito_Scwrl_3EAA.pdb: