Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELLATYLLT-EPGADTEKKAEQIATGLTVGSWTDLPLVKQEQMQKHKGRVIKVEEREGTAASEKQAVITIAYP-----EINFSQDIPALLTTVFGKLSLDGKIKLIDLHFSEAFKRALPGPKFGVYGIRKLLGEFERPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPFETRIAEGKQILKETYEQTGHKTLYAVNLTGRTAD-LKDKARRAAELGADALLFNVFAYGLDVMQGLAE-DPEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFPSPYGSVALPRADALAIHEECVREDA----------FNQTFA-------VPSAGIHPGMVPLLMRDFGIDHIINAGGGVHGHPNGAQGGGRAFRAIIDAVLEAQP------------IDEKAEQCKDLKLALDKWGKAEAV 2WVW Chain:A ((34-462)) -TDLLAAFRFSPQPGVPADEAGAAIAAESSTGTWTTVWTDLLTDMDRYKGKCYHIEPVQG---EENSYFAFIAYPLDLFEEGSVTNILTSIVGNVFGFKAIR-SLRLEDIRFPVALVKTFQGPPHGIQVERDLLNKYGRPMLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENINSQPFQRWRDRFLFVADAIHKSQAETGEIKGHYLNVTAPTCEEMMKRAEFAKELGMPIIMHDFLTAGFTANTTLAKWCRDNGVLLHIHRAMHAVIDRQRNHGI-HFRVLAKCLRLSGGDHLHSGTVVGKLEGDKASTLGF-VDLMREDHIEADRSRGVFFTQDWASMPGVLPVASGGIHVWHMPALVEIFGDDSVLQFGGGTLGHPWGNAPGATANRVALEACVQARNEGRDLYREGGDILREAGKWSPELAAALDLW------

General information: TITO was launched using: RESULT: Template: 2WVW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2196 -212515 -96.77 -542.13 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -96.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_2WVW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WVW-query.scw PDB file : Tito_Scwrl_2WVW.pdb: