Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKTSLIGIILAFVALSVGMVLKGVSFSALANPAAILIIIAGTISAVVIAFPTKEIKKVPTLFRVLFKENKQLTIEELIPMFSEWAQLARREGLLALEASI-EDVDDAFLKNGLSMAVDG----QSAEFIRDIMTEEVEAMEDRHQAGAAIFTQAGTYAPTLGVLGAVIGLIAALSHMDNTDELGHAISAAFVATLLGIFTGYVLWHPFANKLKRKSKQEVKLREVMIEGVLSVLEGQAPKVIEQKLLMYLPAKDRLKFAEQGEAQNGEKKEEEA 4BMV Chain:F ((61-144)) -------------------------------------------------------------------------------------ADLTRPADLAAVEIRLREDARIGILINNAGMAQSGGFVQQTAEGIERLITLNTTALTRLAAAVAPRFVQSGTGA--IVNIGSVVGF--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 227 -18851 -83.04 -238.62 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain F : 0.55 3D Compatibility (PKB) : -83.04 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_4BMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BMV-query.scw PDB file : Tito_Scwrl_4BMV.pdb: