Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKLIEDITKEQLRTDLPAFRPGDTLRVHVKVVEGNRERIQIFEGVVIKRRGGGISETFTVRKISYGVGVERTFPVHTPKIAKIEVVRYGKVRRAKLYYLRELRGKAARIKEIRR 5GAH Chain:Q ((2-112)) -SNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKE---

General information: TITO was launched using: RESULT: Template: 5GAH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Q - contact count / total energy / energy per contact / energy per residue : 414 -33010 -79.73 -297.38 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain Q : 0.83 3D Compatibility (PKB) : -79.73 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_5GAH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAH-query.scw PDB file : Tito_Scwrl_5GAH.pdb: