Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDV---MKRSGRQKDSRFIGAEIASLSYPDRLSKKMLRKASFSSRAALVTLTEAWEEAELDD---ADSSRIGLVVGGSNFQQRENFEVY--ERYQDRSGF--ISPAYGLSFMDSDLCGICTDQFGITGLAYTVGGASASGQLAVIHAIQQVLSGEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPENACRPFDQNRDGFIYGESCGALVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDIDYINPHGTGSFIGDEIELKALRACRLSHA-YINATKSITGHGLSAAGIVEIISVLLQMKKSALHPSRNLDHPIDDSFHWVN--EKSISYRIKNALSLSMGFGGMNTAVCIQNIEKCGGES 1G5X Chain:A ((2-404)) ------KRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGMR--SHVWGN--VKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLIAGSGGGSPR--FQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSISSACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACE-FDAMGALST-KYNDTPEKASRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEGAVRCMKMAMHG---VDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHSLGAAGVQEAIYSLLMLEHGFIAPSINIEE-LDEQAAGLNIVTETTDRELTTVMSNSFGFGGTNATLVMRKL-------

General information: TITO was launched using: RESULT: Template: 1G5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2745 -213908 -77.93 -548.48 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -77.93 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_1G5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G5X-query.scw PDB file : Tito_Scwrl_1G5X.pdb: