Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRPKKAGQQKKTESKAIDSLDKKLGGPNRPST 2K9U Chain:B ((1-24)) MASKPEKRVASSVFITLA----------PPRRDV

General information: TITO was launched using: RESULT: Template: 2K9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2 267 133.25 11.10 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 133.25 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.803

(partial model without unconserved sides chains): PDB file : Tito_2K9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K9U-query.scw PDB file : Tito_Scwrl_2K9U.pdb: