Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKLDLQPSEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAP-TSESRLKTIIEQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIK-MNEISDGVVVGSALVRKIEELKDRLISAETRNQALQEFEDYAMAFSGLYSLK 2DZU Chain:A ((7-229)) ------------LIPYLTAGDPDKQSTLNFLLALDEY-AGAIELGIPFSDPIADGKTIQESHYRALKNGFKLREAFWIVKEFRRHS-STPIVLMTYYNPIYRAGVRNFLAEAKASGVNGILVVDLPVFHAKEFTEIAREEGIKTVFLAAPNTPDERLKVIDDMTTGFVYLVSLYGT----EEIPKTAYDLLRRAKRICRNKVAVGFGVSKREHVVSLLKEGANGVVVGSALVKIIGE--------------------------------

General information: TITO was launched using: RESULT: Template: 2DZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1187 -127354 -107.29 -586.88 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -107.29 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_2DZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DZU-query.scw PDB file : Tito_Scwrl_2DZU.pdb: