Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLYGVIGNPIGHSMSPDIHNASLKDLGLDGHYHAFKVEENDLEDAVKGIRALGVQGINVTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGD-KLVGYNTDGEGFVKSLM--KVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRTLEKAKRLTEATPSFHNKEVLSIKEAEERLEQ-YDVIIHTTSV---GMYPNVDDVPLSLQRAASSAVVCDIVYNPIQTALLKEASQKGL-KTLDGVGMFVEQAALSFQLWTGQEPNIEKMRSIVIGKLGGTEC 3SEF Chain:A ((32-290)) ----YAVFGNP---SKSPFIHTLFARQTQQSMIYTAQCVP-DGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVLLKGA---TILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQLAELVAAYGEVKA----QAFEQLKQSYDVIINSTSASLDGELPAIDPVIFS-----SRSVCYDMMYGKGYTVFNQWARQHGCAQAIDGLGMLVGQAAESFMLWRGLRPGTKQI-------------

General information: TITO was launched using: RESULT: Template: 3SEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1381 -37189 -26.93 -150.56 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -26.93 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_3SEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SEF-query.scw PDB file : Tito_Scwrl_3SEF.pdb: