Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFQLDLIKDKVEFFEAASLQELEKKINTQIENNKAIMLRVKSVSHQTLVAEGRILYSAVVHFSAEA 2GND Chain:A ((23-58)) ---------------------------DAQIKNNAVQLTKTDSNGNPVASTVGRILFSAQVHL----

General information: TITO was launched using: RESULT: Template: 2GND.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 59 1181 20.01 32.79 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 20.01 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_2GND.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GND-query.scw PDB file : Tito_Scwrl_2GND.pdb: