Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPRDALISKNRV---KLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKL-ETEDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEGGCQVPIAGYSVL-NGQDEIEMTGLVASPDGKIIFKETVTG--------NDPEEVGKRC------------------AALMADKGAKDLIDRVKRELDEDGK 3EQ1 Chain:B ((19-356)) --RVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIA---------------------FTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIQGNLNTRLRKLDEQQEFSAIILATAGLQRMGW-HNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQ--LYLTGGVWSLDGSDSIQETMQATIHVPQHEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILD-VARQ------

General information: TITO was launched using: RESULT: Template: 3EQ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1495 3815 2.55 13.57 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 2.55 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_3EQ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EQ1-query.scw PDB file : Tito_Scwrl_3EQ1.pdb: