Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAKRQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTFEYC-KNRKYTPKGEEFDKA--VEEWK-ALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDKKEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRA----IVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSM-NNDVVPEGER--CASTSNRNFEGRQGKGARTH--LVSPAMAAMAAIHGHFVDVRKFYQEKTVV 1AMJ Chain:A ((144-476)) ---------------------------------------------------------------------------------------------------------GIIHQIILE-NYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSV-------FPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGCHYDQLIEINLSELKPHINGPFTPDLAHPV-AEV-------------GSVAEKEGWPL-------DIRVG--LIGSCTNSSYEDMGRSAAVAK-QALAHGLKCKSQFTITPGSEQIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAG---------------

General information: TITO was launched using: RESULT: Template: 1AMJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1781 -39193 -22.01 -122.48 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -22.01 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_1AMJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AMJ-query.scw PDB file : Tito_Scwrl_1AMJ.pdb: