Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQDYSSMMYWAGKHLARKFPLE-----------SWEEFPAFFEEAGWGT-----LTNVSA--KKQELEFELEGPIISNRLKH----QKEPCFQLEAGFIAEQIQLMNDQIAESYEQVKK--RADKVVLTVKWDMKDPV 1SZ7 Chain:A ((49-179)) -------------------------------------VNKQLDKMGFNIGVRLIEDFLARSNVGRCHDFRETADVIAKVAFKMYLGITPSITNWSPAGDEFSLILENNPLVDFVELPDNHSSLIYSNLLCGVLRGALEM-VQMAVEAKFVQDTLKGDGVTEIRMRFIRR---

General information: TITO was launched using: RESULT: Template: 1SZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 14615 43.89 136.58 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 43.89 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_1SZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SZ7-query.scw PDB file : Tito_Scwrl_1SZ7.pdb: