Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKDQLVNEGIRAREVRLIGQNGDQLGIKSRQEALEIAGRANLDLVLVAANAKPPVCRIMDYGKFRFEQQKKEKEARKNQKIINLKEVRLSPTIDEHDFNTKLRNAIKFLEKGDKVKASIRFKGRAITHKEIGQRVLDRFSEACAEVATVETKPKMDGRSMFLMLAPKNEKQ 1ZPD Chain:A ((191-248)) -----------------------------------------------------------------------------------------------DEASLNAAVDETLKFIANRDKVAVLVGSKLRAAGAEEAAVKFTDALGGAVATMAAAKS--------------------

General information: TITO was launched using: RESULT: Template: 1ZPD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 90 -8325 -92.50 -143.53 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -92.50 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_1ZPD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZPD-query.scw PDB file : Tito_Scwrl_1ZPD.pdb: