Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDVQHIDHSFTIGKKGRENEVPVLKDVSLSVAKGEIACIVGRSGSGKSTLLNLISGYISPTKGRIVINGTDVTGFNEKEWAQFRLDHFGFIFQSFQLIPGLTTYENVEM-PLALKGIKPSERKQKVQDMLKRVGLENHAAHYPNELSGGQQQRVSIARALILNPSIILADEPTGSLDSETEHEVLELIQQLNRERGITFVIITHDDEVA-SIGHSKFQLHDGVLKGGITVEV 4YMV Chain:J ((16-214)) -----------------------VLKGVTLKVNKGEVVVIIGPSGSGKSTLLRCINLLEEPTKGEVFIDGVKIN--NGKVNINKVRQKVGMVFQHFNLFPHLTAIENITLAPVKVKKMNKKEAEELAVDLLAKVGLLDKKDQYPIKLSGGQKQRLAIARALAMQPEVMLFDEPTSALDPEMVKEVLNVMKQLANE-GMTMVVVTHEMGFAREVGDRVIFMDDGVI--------

General information: TITO was launched using: RESULT: Template: 4YMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 963 -70733 -73.45 -359.05 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain J : 0.80 3D Compatibility (PKB) : -73.45 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_4YMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YMV-query.scw PDB file : Tito_Scwrl_4YMV.pdb: