Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLWELFVAAILGIVEGLTEYAPVSSTGHMIIVDDIWLKSSNLMSEEAANSFKVVIQLGSILAVAIVFKDRILNLLGLKKNITSDQEQGHKLSIAQIAVGLVPAAVLGFLFEDYIDEYLFSVKTVAIGLIAGAILMLFADWVNKRKTATDTLDRISYKQAIAVGLFQCLSLWPGFSRSGSTISGGVILGLNHRAAADFTFIMAMPIMMGASFLSLVKHWDSLSSDLMPFFIVGFICAFVVALFVVRFFLRLINKIKLVPFAIYRIILGVILLLIMM 5DXA Chain:A ((289-323)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------RCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIV---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 42 -10059 -239.50 -287.40 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -239.50 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_5DXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DXA-query.scw PDB file : Tito_Scwrl_5DXA.pdb: