Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPMIEFCVSNLA-HGSQEARAILE-KDPN-L-DVLEYGCLSYCGTCMESLFALVNGEVVMG-------ETPAELVENIYTFIEENPMF 2FA8 Chain:A ((7-85)) -RIAIRYCTQCNWLLRAGWMAQEILQTFASDIGEVSLIP-----S-TGGLFEITVDGTIIWERKRDGGFPGPKELKQRIRDLIDPE---

General information: TITO was launched using: RESULT: Template: 2FA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 238 -2001 -8.41 -29.43 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -8.41 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_2FA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FA8-query.scw PDB file : Tito_Scwrl_2FA8.pdb: