Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIKEHLKQLKPYQPGKPIEAVKSEYGLDKVVKLASNENPYGCSEAAKEALHHEIQQLAL--YPDGYSAALRTRLSKHLNV-SETSLIFGNGSDEIIQIICRAFLNNKTNTITAAPTFPQYKHNAVIEGAEVREIALRPDGSHDLDAMLEAIDE-QTQVVWICSPNNPTGTYTSEGELLAFLERVPS---RVLVVLDEAYYEYVTAEDYPETVPLLSKYSNLMILRTFSKAYGLAALRVGYGIADENLIRQIEPAREPFNTSRLGQAAAIAALDDQAFIASCVEQNNAGLQQYYDFAKTH-GLKCYPSQTNFVLIDFKRPADELFQALLEKGYIVRSGNALG--FPTSLRITIGTKEQNEEILAILAEIL 3EUC Chain:A ((26-365)) ---------------------VPDSHGL---VKLDAMENPYRLPPALRSELAARLGEVALNRYPVPSSEALRAKLKEVMQVPAGMEVLLGNGSDEIISMLALAAARPGAKVMAPVPGFVMYAMSAQFAGLEFVGVPLRADFTLDRGAMLAAMAEHQPAIVYLAYPNNPTGNLFDAADMEAIVRAAQGSVCRSLVVVDEAYQPFAQ-ESWMSR---LTDFGNLLVMRTVSK-LGLAGIRLGYVAGDPQWLEQLDKVRPPYNVNVLTEATALFALEHVAVLDEQAAQLRAERSRVAEGMAAHGGVTVFPSAANFLLARVPDAA-QTFDRLLARKVLIKNVSKMHPLLANCLRVTVSTPEENAQFLEAFAASL

General information: TITO was launched using: RESULT: Template: 3EUC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1722 -5885 -3.42 -17.83 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -3.42 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_3EUC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EUC-query.scw PDB file : Tito_Scwrl_3EUC.pdb: