Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYEEHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVTGASTIAKYKMSFGELELKTSTKSIQSDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA 3IG9 Chain:A ((5-49)) ---------------------------------------------------------------------------------------------VNIKTFTHPAGEGKEVKGMEVSVPFEIYSNEHRIADAHYQTFPSE----

General information: TITO was launched using: RESULT: Template: 3IG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 103 -4443 -43.13 -98.72 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -43.13 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.041

(partial model without unconserved sides chains): PDB file : Tito_3IG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IG9-query.scw PDB file : Tito_Scwrl_3IG9.pdb: