Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLTDKTVLITGGASGIGYAAVQAFLNQQANVVVADIDEAQGEAMIRKENNDRLH--FVHTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAP----IHEMELSNWNKVLNVNLTGMFLMSKHALKYMLKSGKGNIINTCSVGGVV--AWPDIPAYNASKGGVLQLTRSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVN-PLLRLGKPEEIANVMLFLASDLSSYMTGSAITADGGYTAQ 4WEC Chain:A ((27-272)) -RLAGKVAVITGGASGIGLATGRRLRAEGATVVVGDIDPTTGKAA-----ADELEGLFVPVDVSEQEAVDNLFDTAASTFGRVDIAFNNAGIS--PPEDDLIENTDLPAWQRVQDINLKSVYLSCRAALRHMVPAGKGSIINTASFVAVMGSATSQI-SYTASKGGVLAMSRELGVQYARQGIRVNALCPGPVNTPLLQELFAKDPE------RAARRLVHIPLGRFAEPEELAAAVAFLASDDASFITGSTFLVDGGISS-

General information: TITO was launched using: RESULT: Template: 4WEC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1300 -32645 -25.11 -137.74 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -25.11 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4WEC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WEC-query.scw PDB file : Tito_Scwrl_4WEC.pdb: