Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINIVTIGKLKEKYLKQGIEEYTKRLSAYAKIDIIELPDEKAPENLSDQDMKIIKDKEGDRILSKISPDAHVIALAIEGK--------MKTSEELADTIDKLATYGKS---KVTFVIGGSLGLSD---TVMKRADEKLSF----SKMTFPHQLMR-----------LILVEQIYRAFRINRGEPYHK 4WWS Chain:A ((83-253)) ------ALNEVENRFNKLAIADYL--LPTYSYISVVELSNYLASHMAGGDDPYQNKGVRA-RLYPALPPKKHICFYPMSKKRDGADNWYMLPMEERQQLIRDHGLIGRSYAGKVQQIIGGSIGFDDYEWGVTLFSDDALEFKRIVTEMRFDEASARYAEFGSFFIGNLLLSEQLSKLFTI--------

General information: TITO was launched using: RESULT: Template: 4WWS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 379 -1386 -3.66 -9.76 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -3.66 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_4WWS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WWS-query.scw PDB file : Tito_Scwrl_4WWS.pdb: