Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRRLAIVQRWRGQDEIPTDWGRCVLTIGVFDGVHRGHAELIAHAVKAGRARGVPAVLMTFDPHPMEVVYPGSHPAQLTTLTRRAELVQDLGIEVFLVMPFTTDFMK---LTPDRFIHELLVEHLHVVEVVVGENFTFGKKAAGNVDTLRRAGERFGFAVESMSLVSEHHSNETVTFSSTYIRSCVDAGDMVAAMEALGRPHRVEGVVVRGEGRGAELGFPTANVAPPMYSAIPAD-GVYAAWFTVLGHGPVTGTVVPGERYQAAVSVGTNPTFSGRTRTVEAFVLDTTADLYGQHVALDFVGRIRGQKKFESVRQLVAAMGADTERARDLLSTG 1S4M Chain:A ((1-282)) -----------------------MVVSIGVFDGVHIGHQKVLRTMKEIAFFRKDDSLIYTI-SYPPEYFLP-DFPGLLMTVESRVEMLSRYARTVVL------DFFRIKDLTPEGFVERYLS---GVSAVVVGRDFRFGKNASGNASFLRKKGVEV-YEIEDVVVQGKR-------VSSSLIRNLVQEGRVEEIPAYLGRYFEIEGIVHK---------FPTANIDRGNEKLVDLKRGVY----LVRVHLP------DGKKKFGVMNVGFRPT-DARNVKYEVYILDFEGDLYGQRLKLEVLKFMRDEKKFDSIEELKAAIDQDVKSARNMID--

General information: TITO was launched using: RESULT: Template: 1S4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -187326 for 2057 contacts (-91.1/contact) + 2D Compatibility (PS) -28301 + (NN) -4009 + (LL) 3572 1D Compatibility (HY) -24800 + (ID) 4750 Total energy: -245614.0 ( -119.40 by residue) QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_1S4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S4M-query.scw PDB file : Tito_Scwrl_1S4M.pdb: