Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTVPQNMKAAVMHNT-REIKIETLPVPDINHDEVLIKVMAVGICGSDLHYYTNGRIGNYVVEKPFILGHECAGEIAAVGSSVDQFKVGDRVAVEPGVTCGRCEACKEGRYNLCPDVQF-LA-----------------------TPPVDGAFVQYIKMRQDFVFLIPDSLSYEEAALIE-PFSVGIHAA-ARTKLQPGSTIAIMGMGPVGLMAVAAAKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQD--ALEEIKTITNDRGVDVAWETAGNPAALQSALASVRRG-GKLAIVGLPSQNEIPLNVPFIADNEIDIYGIF----RYANTYPKGIEFLASGIVDTKHLVTDQYSLEQTQDAMERALQFKNECLKVMVYPNR 1E3I Chain:A ((4-368)) ---GKVIKCKAAIAWKTGSPLCIEEIEVSPPKACEVRIQVIATCVCPTDINATDPKKK----ALFPVVLGHECAGIVESVGPGVTNFKPGDKVIPFFAPQCKRCKLCLSPLTNLCGKLRNFKYPTIDQELMEDRTSRFTCKGRSIYHFMGVSSFSQYTVVSEANLARVDDEANLERVCLIGCGFSSGYGAAINTAKVTPGSTCAVFGLGCVGLSAIIGCKIAGASRIIAIDINGEKFPKAKALGATDCLNPRELDKPVQDVITELTA-GGVDYSLDCAGTAQTLKAAVDCTVLGWGSCTVVGAKVD-EMTIPTVDVI-LGRSINGTFFGGWKSVDSVPNLVSDYKNKKFDLDLLVTHALPFESINDAIDLMKEGK------------

General information: TITO was launched using: RESULT: Template: 1E3I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1993 -249104 -124.99 -752.58 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -124.99 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_1E3I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E3I-query.scw PDB file : Tito_Scwrl_1E3I.pdb: