Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGPAAGSRARVYAEVNSLRSREYWDYEAHVPSWGNQDDYQLVRKLGRGKYSEVFEAINITNNERVVVKILKP-----VKKKKIKREVKILENLRGGTNIIKLIDTVKDPVS----KTPALVFEYINNTDFKQLYQILTDFDIRFYMYELLKALDYCHSKGIMHRDVKPHNVMIDHQQKKLRLIDWGLAEFYHPAQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRREPFFHGQDNYDQLVRIAKVLGTEELYGYLKKYHIDLDPHFNDILGQHSRKRWENFIHSENRHLVSPEALDLLDKLLRYDHQQRLTAKEAMEHPYFYPVVKEQSQPCADNAVLSSGLTAAR 2FST Chain:X ((23-322)) -------------------------------TIWEVPERYQNLSPVG----GSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMK-HENVIGLLDVFTPARSLEEFNDVYLVTHLM-GADLN---QKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNED-CELKILD-----------------ATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTL-FPGTDHIDQLKLILRLVGTPGAE-LLKKISSESARNYIQSLTQMPKMNFANVFI-----GANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSLESRDLL

General information: TITO was launched using: RESULT: Template: 2FST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -151509 for 1972 contacts (-76.8/contact) + 2D Compatibility (PS) -28318 + (NN) -11175 + (LL) 3844 1D Compatibility (HY) -24800 + (ID) 4250 Total energy: -216208.0 ( -109.64 by residue) QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_2FST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FST-query.scw PDB file : Tito_Scwrl_2FST.pdb: