Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKQTSGKYEKILQAAIEVISEKGLDKASISDIVKKAGTAQGTFYLYFSSKNALIPAIAENLLTHTLDQIKGRLHGDEDFWTVLDILIDETFLITERHKDIIVLCYSGLAIDHSME-KWETIYQPYYSWLEKIINKAIANHEVTEGINSKWTARTIINLVENTAERFYIGFEQDENVEVYKKEIFTFLKRSLGTA 4ME9 Chain:A ((3-186)) ----AKNKQEDIFDAAMQLFAERGYDGTTIPMIAEKAKVGAGTIYRYFENKEALVNSLFSKSMLQLSEMIKTDFPVEANIREQFSHTYNRLFEFARNNVDAFLFTNS-HCDSYFLDEQSKKIFDDFIGFFMNIIEDGIVKGLLRP-LPPVALIIIVYQPLEKLIKVIATGQL--EYSKELVKELEESSWNAI---

General information: TITO was launched using: RESULT: Template: 4ME9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 639 -53478 -83.69 -292.23 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -83.69 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_4ME9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ME9-query.scw PDB file : Tito_Scwrl_4ME9.pdb: