Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKIIAITNQKGGVGKTTTSVNLGACLAY-IGKRVLLVDID-PQGNATSGLGIEKADVEQCVYDILVDDAD-----VIDIIKATTVENLDVIPATIQLAGAEIELVPTISREVRLKRALEAVKQNYDYIIIDCPPSLGLLTINALTASDSVVIPVQCEYYALEGLSQLLNTVRLVQKHLNTDLMIEGVLLTMLDARTNLGIQVIEEVKKYFRDKVYKTVIPRNV-RLSEAPSHGKPIILYDPRSRGAEVYLDLAKEVAANG 3EA0 Chain:A ((3-242)) AKRVFGFVSAKGGDGGSCIAANFAFALSQEPDIHVLAVDISLPFGDLDMYLSGNTHS--QDLADISNASDRLDKSLLDTMVQ-HISPSLDLIPSPATFEKIV-NI-----EPERVSDLIHIAASFYDYIIVDFGASIDHVGVWVLEHLDELCIVTTPSLQSLRRAGQLLKLCKEFEK----PISRIEIILNRADTNSRIT---SDEIEKVIGRPI-SKRIPQDEDAMQESLLSGQSVLKVAPKSQLSKTIVDWALHL----

General information: TITO was launched using: RESULT: Template: 3EA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1269 -27715 -21.84 -119.46 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -21.84 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_3EA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EA0-query.scw PDB file : Tito_Scwrl_3EA0.pdb: