Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSFMHSKAVIFSFTMAFFLILAACSGKNEADSKDTGWEQIKDKGKIVVATSGTLYPTSYHDTDSGSDKLTGYEVEVVREAAKRLGLKVEFKEMGIDGMLTAVNSGQVDAAANDIDVTKDREEKFAFSTPYKYSYGTAIVRKDDLSGIKTLKDLKGKK-AAGAATTVYMEVARKYGAKEVIYDNATNEQYLKDVANGRTDVILNDYYLQTLALAAFPDLNITIHPDIKYMPNKQ-ALVMKKSNAALQKKMNEALKEMSKDGSLTKLSKQFFNKADVSKKIDADVQDVDL 4PSH Chain:A ((3-225)) --------------------------------------DEIKSRGYLLVGLSADFPPFEFVDENG---NIVGFDVDLAKEIARRLGVELKIVDMTFDGLIPSLLTKKIDVIISGMTITEERKKVVAFSDPYFDAGQVIVVRKDSDFRPKTYEDLVGKTVAVQIGTTGDIEVSKYDGIKVVRFDKFTDA--FLELKRGRADAVVLDSATARAFVAKNPDLVISSG----VLSSEQYGIAVRKEDTDLLEFINSVLRELKKSPYDVLIEKWF-------------------

General information: TITO was launched using: RESULT: Template: 4PSH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1110 -103781 -93.50 -469.60 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -93.50 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4PSH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PSH-query.scw PDB file : Tito_Scwrl_4PSH.pdb: