Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTSPRMKWFVLLFTFVFAIGMNSFRNSFQFFMLPMADAFH--------------ADRSLISVSVSIFMITTGIVQFFVGFFIDRFSVRKIMALGAVCISASFLVLPY---SPNVHVFSAIYGVLGGIGYSCAVGVTTQYFISCWFDTHKGLALAILTNANSAGLLLLSPIWAAAP--YHAGWQSTYTILGIVMAAVLLPLLVFGMKHPPHAQA--ETVK--K--------------------------SYDWRGFWNVMKQSRLIHILYFGVFTCGF-----TMGIIDAHLVPILKDAHVSHVNGMMAAFGAFIIIGGLLAGWLSDLLGSRSVMLSILFFIRLLSLICLLIPI-LGI--HH-SDLWYFGFILLFGLSYTGVIPLTAASISESYQTGLIGSLLGINFFIHQVAGALSVYAGGLFFDMTHGYLLIVAVCIVFVGLSAVIELVPFLDKQKAKETHHSI 4ZW9 Chain:A ((51-450)) --------------------------GVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLIL-GRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAIL-QSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMI-GLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLG-------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1571 -256943 -163.55 -751.30 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -163.55 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: