Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MRNQMKIALLVGLAIVCIGLFLFYDLGNWDYTLPRRIKKVAAIVLTGGAIAFSTMIFQTITNNRI-----LTPSILGLDSLYMLIQTGII-FLFGSANMVIMNKNINFIISVL--------LMILFSLV----LYQIMFKGEGR--NIFFLLLIGIVFG--TLFSSLSSFMQMLIDPNEFQVVQDKMFASFNNINT---------DLLWLAFIIFLLTG------VYVWRFTKFFDVLSLGREHAVNLG----IDYDKVVKQ-------------MLIVVAILVSVSTALVGPIMFLGLLVVNLARE---FL------KTYKHSYLIA-----GSVFISIIALVGGQFV---------VEKVFTFSTTLSVIINFAGGIYFIYLLLKENKSW----------- 4CZB Chain:A ((1-415)) MELMMAIGYLGLALVLGSLVAKIAEKLKIPDIPLLLLLGLIIGPFLQIIP--SDSAMEIFEYAGPIGLIFILLGGAFTMRISLLKRVIKTVVRLDTITFLITLLISGFIFNMVLNLPYTSPVGYLFGAITAATDPATLIPVFSRVRTNPEVAITLEAESIFNDPLGIVSTSVILGLFGLFSSSNPLIDLITLAGGAIVVGLLLAKIYEKIIIHCDFHEYVAPLVLGGAMLLLYVGDDLLPSICGYGFSGYMAVAIMGLYLGDALFRADDIDYKYIVSFCDDLSLLARVFIFVFLGACIKLSMLENYFIPGLLVALGSIFLARPLGVFLGLIGSKHSFKEKLYFALEGPRGVVPAALAVTVGIEILKNADKIPASITKYITPTDIAGTII--IGTFMTILLSVILEASWAGMLALKLLGE

General information: TITO was launched using: RESULT: Template: 4CZB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1390 -310229 -223.19 -997.52 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -223.19 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.230

(partial model without unconserved sides chains): PDB file : Tito_4CZB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZB-query.scw PDB file : Tito_Scwrl_4CZB.pdb: