Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTNYWIGVVSEQHVLK--GAAGG----FAQLC--HGKKAPLAKMKEGDWLIYYSPRDAYPDGKLLRSFTAIGKVKSGNIYPYQMAPNF-IPYRLDIDYYPC--HKIGFYDIKSK-----LEFVQETKHLGFLFRRGHFEISKKDFLTIAQAMGVNISGMAL 1ZCE Chain:A ((2-140)) --ANYWLYKSEPFKWSWEMQKAKGETGEEWTGVRNYQARNNMRAMKIGDKGFFYHSNEG-------LDVVGIVEVCA-LSHPDSTAEGDLKWDCVDIRAVCDMPQPVSLKDVKANPKLEKMSLVTS-------MRLSVQPVTEEEYLEVCRMGGLA------

General information: TITO was launched using: RESULT: Template: 1ZCE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 589 -67247 -114.17 -546.72 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -114.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_1ZCE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZCE-query.scw PDB file : Tito_Scwrl_1ZCE.pdb: