Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRVNATIITDVEALKADKDLNHFKDYATCETFVV------DADNHLKANEEISE-------LLKGWDWWCIWSTRPLSKKDNQVF--TLYNDDLYNLCR 2LUW Chain:A ((1-101)) GNVLKNNTPVSNLTGNKGSEVFYTFTVDRNATAVVSISGGSGDADLYLKAGSKPTTSSWDCRPYRYGNNESCSVSAAPGTTYHVMIKGYSNYSGVTLKLQY

General information: TITO was launched using: RESULT: Template: 2LUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 349 -6861 -19.66 -79.77 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -19.66 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.60 QMean score : -0.063

(partial model without unconserved sides chains): PDB file : Tito_2LUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LUW-query.scw PDB file : Tito_Scwrl_2LUW.pdb: