Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYIILEDQTRLYYETHGSGTPILFIHGVLMSGQFFHKQFSVLSAN-YQCIRLDLRGHGESDKVLHGHTISQYARDIREFLNAMELDHVVLAGWSMGAFVVWDYLNQFGNDNIQAAVIIDQSASDY-QWEGWEHG-PFDF------DGLKTAMHAIQTDPLPFYESFIQNMFAEPPAETETEWMLAEILKQPAAISSTILFNQTAADYRGTLQNINVPALLCFGEDKKF--FSTAAGEHLRSNIPNATLVTFPKSSHCPFLEEPDAFNSTLLSFLDGVIGKS 3T4U Chain:A ((4-269)) ----VAKDGTQIYFKDWGSGKPVLFSHGWILDADMWEYQMEYLSSRGYRTIAFDRRGFGRSDQPWTGNDYDTFADDIAQLIEHLDLKEVTLVGFSMGGGDVARYIARHGSARVAGLVLLGAVTPLFGQKPDYPQGVPLDVFARFKTELLKDRAQFISDFNAPFY-GINKGQVVSQGVQTQT-LQIALLASLKATVDCVTAFAET--DFRPDMAKIDVPTLVIHGDGDQIVPFET-TGKVAAELIKGAELKVYKDAPHGFAVTHAQQLNEDLLAFL-------

General information: TITO was launched using: RESULT: Template: 3T4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1521 -175916 -115.66 -689.87 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -115.66 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_3T4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T4U-query.scw PDB file : Tito_Scwrl_3T4U.pdb: