Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MANSNNKTNAQQVRKQNQQSASGQG------QFGTEFASETNVQ-------QVRKQNQQSAAGQ-------GQFGTEFASETDAQQVRQQNQSAEQNKQQNS 2MHQ Chain:A ((1-103)) GAMGSCRPPMVKLVCPADNLRAEGLECTKTCQNYDLECMSMGCVSGCLCPPGMVRHENRCVALERCPCFHQGKEYAPGETVKIGCNTCVCRDRKWNCTDHVCD

General information: TITO was launched using: RESULT: Template: 2MHQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 123 4475 36.38 54.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 36.38 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_2MHQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MHQ-query.scw PDB file : Tito_Scwrl_2MHQ.pdb: