Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTDLTFIHAADLHLDSPFYGISHLPEPIFARIKESTFASVRHMIDAAVREHVDFILLAGDLF-DEANRSLKAQLFLKKQFERLRECGISVYVIFGNHDHLGGEWTPIEWPENVHIFSSAVPEEKSFF----------KEGRRIASIYGFSY--QARALMENQA-------ARYRRSTDAP-----FHIGMLHGTLSGSEGHDPYCP----FTHDDLVKSGMDYWALGHIHK-RQVLSAEHPAVIYPGNTQARHIKETGD-KGYYLVHVTNGDISYEFQRAHDVLWEKAAVDVTEAKNMTALFQMVEDTFSKLRKKGSPVCVRLVLQGTAPEWLLEAPKGTLDEFLEALQEQEAEEERFVWPLRLDDETENEANLTNLDPFFGGLFEDIDRSDLSDVLEGLERHPVYRRHADRFSQEEVKEIKEQAQIILKRQLKVLDT 4O43 Chain:A ((16-270)) -LKELKILHTSDWHLGVTSWTSSR---PVDRR--EELKKALDKVVEEAEKREVDLILLTGDLLHSRNNPSVVALHDLLDYLKRMMRTA-PVVVLPGNHD-----WKGLKLFGN---FVTSISSDITFVMSFEPVDVEAKRGQKV-RILPFPYPDESEALRKNEGDFRFFLESRLNKLYEEALKKEDFAIFMGHFTVEGLAGYAGIEQGREIIINRALIPSVVDYAALGHIHSFREI--QKQPLTIYPGSLIRIDFGEEADEKGAVFVELKRGE----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4O43.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1062 -136306 -128.35 -608.51 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -128.35 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_4O43.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O43-query.scw PDB file : Tito_Scwrl_4O43.pdb: