Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQDMILTLQKHWSSQGCVLMQAYDVEKGAGTMSPYTFLRSIGPEPWKVAYVEPSRRPADGRYGENPNRLYQHHQFQVIIKP---SPDNIQELYLDSLRALGIDPLEHDIRFVEDNWENPSLGCAGLGWEVWLDGMEITQFTYFQQV----GGLECKPVSVEITYGIERLASYIQDKENVFDLEWTSGFTVKDLFMMAEYEHSVYTFETSDVDMLFQLFSTYEKEAIKQMDNGLVHPAYDYVLKCSHTFNLLDAKGAISVTERTGYIARVRNLARKVAKTYYEEREKLGFPMLKGEGSSHE 4CS4 Chain:A ((131-256)) --------------------------------------------PDPIKIFEIGPCYRKESDG----KEHLEEFTMLNFCQMGSGCTRENLESIITDFLNHLGID-----FKIVGDSCMVF-----GDTLDVMHGDLELSSAVVGPIPLDREWGID-KPWIG-AGFGLERLLKVKHDFKNIKRAAR--------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4CS4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 466 -13678 -29.35 -114.94 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -29.35 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4CS4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CS4-query.scw PDB file : Tito_Scwrl_4CS4.pdb: