Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEKLRLYHTNDLHSHF----ENWPKIVDY-------------IEQKRKEHQSDGEETLVFDIGDHLDRFQFVTEATFGKANVDLLNRLHIDGAAIGNNEGITLPHEELAALYDHAEFPVIVSNLFDKN---GNRPSWAVPYHIKSLKNGMSIAFLGVTVPYYPVYDKLGWTVT--DALESIKETILEVKG-QADIIVLLSHLGILDDQAVAEAVPEIDVILESHTHHLLEDG-----QVVNG-------------VLLASAEKYGHYVGCVEITVDSVQRSINSKTASV---QNMAEWTGESAETKAFLNEKEREAEEKLSDAVAELAQDAEVKWFEESELPLLLAYALKE----------WCETDISMVNSGVILGPL---KAGPVTKLDLHRICPHPINPVAVRLTGEELKETIVHAASEQMEQLRIKGLGFRGEVMGKMVYAGVEVETKRLDDGITHVTRITLNGEDIEKHKQYSVAVLDMFTLGKLFPLIRDAAEKEYFMPEFLRDLLAWKLAQ 4H2I Chain:A ((3-413)) ---ELTILHTNDVHSRLEQTSEDSSKCVDASRCMGGVARLFTKVQQIRRAEPN----VLLLDAGDQYQGTIWFT-VYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEV-VGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLDSSIPEDPSIKADINKWRIKLDDYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADEMFWNHVSMCILNGGGIRSPIDERNDGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHS---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4H2I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2021 23657 11.71 66.83 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 11.71 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_4H2I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H2I-query.scw PDB file : Tito_Scwrl_4H2I.pdb: