Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVIFLQDVKGKGKKGEVKNVADGYAHNFLIKKGLAVEANASNISALNGQKQKEKKEAIAELEQAKSLKETLEKL-TVELSAKSGEGGRLFGSVTSKQITEQLQKDHNIKVDKRKLELPDGI-RALGYTNVPVKLHPEVQAVLKVHVKEEA 3J8G Chain:H ((1-149)) MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTA-AGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE-

General information: TITO was launched using: RESULT: Template: 3J8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 583 876 1.50 5.96 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : 1.50 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.252

(partial model without unconserved sides chains): PDB file : Tito_3J8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J8G-query.scw PDB file : Tito_Scwrl_3J8G.pdb: