Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDS-----IPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM 1H00 Chain:A ((4-297)) MENFQKVEKIGE--YGVVYKARNKLTGEVVALKKI--------VPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARA----------EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL

General information: TITO was launched using: RESULT: Template: 1H00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 32158 for 2121 contacts (15.2/contact) + 2D Compatibility (PS) -26653 + (NN) 8108 + (LL) 1112 1D Compatibility (HY) 18800 + (ID) 450 Total energy: 33075.0 ( 15.59 by residue) QMean score : 0.071

(partial model without unconserved sides chains): PDB file : Tito_1H00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H00-query.scw PDB file : Tito_Scwrl_1H00.pdb: