Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDISSTEIWDAIRRNSYLLYYQPKVDAKTNKIIGFEGLVRLKTATTILAPIDFFDDI-----VLLNATREMQDFVAETAIKQINQLGGRFSISINIPAHYVASSTY----MTFLHDYVKEHLKYPECLEIEIIERGEITELAIADKNLRKIKDLGVKVSMDDFGKGYSSLAYLRSLPIDIVKTDMSFIALLKTDRKQQIIIRAIVNLCHDLGGKVVTEGVEDMEQVEKLREMKVDYFQGYYFSRPLPMEEIKQKYSIV 4KIE Chain:A ((22-254)) ----------ALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVNKLGNDKIK----LVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVP-----------

General information: TITO was launched using: RESULT: Template: 4KIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -97024 for 1714 contacts (-56.6/contact) + 2D Compatibility (PS) -24002 + (NN) -7638 + (LL) 2160 1D Compatibility (HY) -18400 + (ID) 3200 Total energy: -148104.0 ( -86.41 by residue) QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4KIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KIE-query.scw PDB file : Tito_Scwrl_4KIE.pdb: