Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQ---------KLLLSGLAVSTV----GITSYLLKDP----SNRQKAREFIHSMK--MKITKQPDMETFPVD---KAGHPDPQDIEDNKMVSEGSMYPVQYYDEKKK 2H2M Chain:A ((1-108)) MAAGELEGGKPLSGLLNALAQDTFHGYPGITEELLRSQLYPEVPPEEFRPFLAKMRGILKSIASADMDFNQLEAFLTAQTKKQGGITSDQAAVISKFWKSHKTKIRES

General information: TITO was launched using: RESULT: Template: 2H2M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 229 -18486 -80.72 -214.95 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -80.72 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_2H2M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H2M-query.scw PDB file : Tito_Scwrl_2H2M.pdb: