Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRLHYAWIIVSVTFLILLAVQGVRLSFGAFV-EPWERQFSIDRSTISLISTVSFIVYGISQPVIGRLVDKW-GARAVLAWSALLTGVSIFLTYLVT-SPWQLFLLYGLGVSLGVGGASNVAASVLVVNWF-SK-KRGLAFGIMEAGFGAGQMLLVPGSLMLIHW--------FSWKLTVVVLGLLLIVIVFPAALLMLRNNPSEKNTEPIGGLAAS-EK----------------ETAPKT-----------------T-ALSVAGMFRMRQ---FWFLIFPFLICGFTTVGLMDTHLIPFSHD-HGFS---------TTVTSAAVSLLAGFNIAGILLSGIVADRWSS-RKILCILYAVRALSIVILIYS--------HEPYLLLAFAILFGLVDFATVAPTQMLATQYFQNYSIGLMIGWLSLAHQIGSALGAYVPGVIYTVTGEYTLAFYLSIGMLVLASVMNVMLREPAAVTRDSAAVVDK 4TPH Chain:A ((30-451)) --------------------------GMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSAL-GVIVVGNGLF-KPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGI-LVGLGN-YALMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILEYEDVARVFVYAAGVAVLGIFFHL-------ERAGLIAALILTVQTVFFFIFYQQM-STSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR---------MMGAYFVASGISQYLGGVVANFASVPQ----------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1474 -223175 -151.41 -697.42 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -151.41 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: