Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAPSIKSGT--ILHAWNWSFNTLKHNMKD-IHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQPTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAI-------------SNEVKSIP----NWTHGNTQ-----IKNWSDRWDVTQNSLLGLYDWNTQNTQVQSYLKRFLDRALNDGADGFRFDAAKHIELPDDGSYGSQFWPNITN----TSAEFQYGEI--LQDSASRDAAYANYMDVTASNYGHSIRSALKN----RNLGVSNISHYASDVSADKLVTWVESHDT-YANDDEESTWMSDDDIRLGWAVIASRSGSTPLFFSR-----------------------PEGGG--NGVRFPGKSQIGDRGSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSINTATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVTHSFNDQLTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTIMLKGTNSDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMTKNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH 1BVN Chain:P ((3-451)) -------------------------------------------APQTQSGRTDIVHLFEWRWVDIALECERYLGPKGFGGVQVSPPNENVVVTNPSRP---WWERYQPVSYKLCTRS-GNENEFRDMVTRCNNVGVRIYVDAVINHMCGSGAAAGTGTTCGSYCNPGSREFPAVPYSAWDFNDGKCKTASGGIESYNDPYQVRDCQLVGLLDLALEKDYVRSMIADYLNKLIDIGVAGFRLDASKHMWPGDIKAVLDKLHNLNTNWFPAGSRPFIFQEVIDLGGEAISSSEYFGNGRVTEFKYGAKLGTVVRKWSGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGSSILTFWDARL-YKVAVGFMLAHPYGFTRVMSSYRWARNFVNGEDVNDWIGPPNNNGVIKEVTINADTTCGNDWVCEHRWREIRNMVWFRNVVDGQPFANWWDNGSNQVAFG-RGNRGFIVFNNDDWQLSSTLQTGLPGGTYCN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1BVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 2069 -105888 -51.18 -272.91 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain P : 0.64 3D Compatibility (PKB) : -51.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_1BVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BVN-query.scw PDB file : Tito_Scwrl_1BVN.pdb: