Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------MFNINLLNQARLESDLDSLGCLDIAEELIEKMMEDVFYSDVAQRIIVQTLAVINNANAKLQATFNILEDEDMETDERSVEFEGISYYE------------------------------------------------------------------------ 4OLK Chain:A ((4-167)) MAKTQAEINKRLDAYAKGTVDSPYRIKKATSYDPSFGVMEAGAIDADGYYHAQ---------CQDLITDYVLWLTD-------------NKVRTWGNAKDQIKQSYG----------------TGFKIHENKPSTVPKKGWIAVFTSGSYQQWGHIGIVYDGGNTSTFTILEQNWNGYANKKPTKRVDNYYGLTHFIEIPVK

General information: TITO was launched using: RESULT: Template: 4OLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 -8446 -65.98 -168.92 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -65.98 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_4OLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OLK-query.scw PDB file : Tito_Scwrl_4OLK.pdb: