Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFILNENKQLSLAIEDENIENFYIDGTDLVRKIIRRSGSGVTSRVPVLSTQDLENKNLHELYDESWLRMKNRPNTELTTESINIADLFSGCGGLSLGVWEACRALGINPRFSFACDLNEAALSVYEKNFSPDFSLNESIEKHINGELGAPLTVEEQRIKDKVKKIDFILAGPPCQGHSDLNNHTRRKDPRNALLMRVSRVIELFQPSSVLVENVPGII-HDKSGSFKEFKNHLKTQGYYFDEIVLNAEKLGVSQARRRYFIFASKTPVSSLNQINEFYSTNSRPISWAISDLVENVGDDIFNTASEHSLENKRRIEYLFENNLFELPNSERPDCHRLKPHSYKSVYGRMYWDRPAPTITRGFGS---TGQGRFVHSLLKRTITPHEAARIQFFPDFFNFGDLRRRQYQDVIGNAVPSKLSYLLALHQLR 8MHT Chain:A ((16-314)) ---------------------------------------------------------------------------------------DLFAGLGGFRLAL-ESCGAECV-----YSNEWDKYAQEVYEMNFG------EKPEGDIT-------QVNEKTIPDH----DILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIPQKRERIYMICFRNDLN----IQNFQFPKPFELNTFVKDLL------LPDSEVEHLVIDRKDL--VMTNQEIE---QTTPKTVRLGIVGKGGQGERIYSTRGIAITLSAYGGGIFAKTGGYLVNGKTRKLHPRECARVMGYPDSYKVHPSTSQAYKQFGNSVVINVLQYI-------

General information: TITO was launched using: RESULT: Template: 8MHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1436 -105798 -73.68 -358.64 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -73.68 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_8MHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-8MHT-query.scw PDB file : Tito_Scwrl_8MHT.pdb: