Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKQIIYPGAVIGIIGGGQLGKMMAVSAKQMGYKVAVVDPVKDSPCGQ-----VADVEITAHYNDREAIRKLAEISDIITYEFENIDYDALHWLKDHAYLPQGSELLLITQNRETEKKAIQSAGCEVAPYSIVKTKNELKQAVQELRLPAVLKTCRGGYDGKGQFVIKEEAQMEQAAALLEHGT--CILESWVSFKMELSVIVVRSVNGEISTFPTAENIHHNNILFQSIVPARVEKGIQQKAADLAVKLADELNLVGPLAVEMFLTEDGELLVNELAPRPHNSGHYTLDLCETSQFEQHIRAVCGLPLGKTDLLKPGMMVNLLGDEVKLVEEDPELLKEAKLYIYGKHEIKKGRKMGHITF-MKQPEDEWIQEITNKWMNRDGGQAE 4MAM Chain:A ((3-350)) -----------IGIIGAGQLARMLSLAGTPLGLEFHCLG--KNGDCAEEVVKTVTDIELT-KVND---VVAWAKQFDVITFENENISHELIKAINHEVSVYPSAKAIAISQDRLLEKSFMQDHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPFE-NVVLAEKAQQIAKILVKEFAYVGTLAIEFFVKGD-ELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTSRKT-VMLNCIGGMP--ATKDLAALDRVKIHSYNK-EPRKGRKVGHLNLNLNDETDEY-----------------

General information: TITO was launched using: RESULT: Template: 4MAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1919 -183045 -95.39 -538.37 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -95.39 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4MAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MAM-query.scw PDB file : Tito_Scwrl_4MAM.pdb: