Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIKKKVMMCLAVTLVFGSMSFPTLTNSGGFK-----ESTDRNTTYIDHSPYKLSDQKKALS 2VGA Chain:A ((42-84)) ------------ASVSNGGLNISVDLLNCFLNFHTVGVYTNRDTVYAKFASLDPW-------

General information: TITO was launched using: RESULT: Template: 2VGA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 77 -8216 -106.69 -216.20 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -106.69 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_2VGA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VGA-query.scw PDB file : Tito_Scwrl_2VGA.pdb: