Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFFKKLAASAGIGAAKVDTILEKDAYFPGEEVQGTVHVKGGKIAQDIRYIDLQLSTRYVIVKDDEE-----HRKYATIHSFRVT-------------GSFTIQPGEEHQFPFTFTLPLDTPITV------------GKVEVAVVTD---LDIQGGIDKSDHDRIFVEAHPWIENVLEAIENLGFRLNEADCEQAPYFQRRLPFVQEFEFVPTSGYYRQMLDELELIFLLDEDGLEIIFEVDRRARGLRGWLEEMYNDGEQLVRVRFSQSELEDTEELEEVLEEILDQYAE 4ZWJ Chain:A ((2035-2185)) -------------------------HVSQVEPVDGVVLVDPE--LVKGKKVYVTLTCAFRYGQEDIDVMGLTFRRDLYFSRVQVYPPVGAMSVLTQLQESLLKKLGD-NTYPFLLTFPDYLPCSVMLQPAPQDVGKSCGVDFEVKAFASDITDPEEDKIPKKSSVRLLIRKVQHAP-------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 393 -41035 -104.41 -356.82 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -104.41 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4ZWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZWJ-query.scw PDB file : Tito_Scwrl_4ZWJ.pdb: