Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -METTKLG----NLKLYGAGHAAGGAYHNVSIK-GEGIVGEGLSAV-GCRIYGTGLFLGKAETERLRVLGES-EC-----KGDLT-AGKINIYGTMKVSGSLQFDRFNLKGQTEIGGNMTGESC-DVKGKLSVIGDCETEMFHVTGCVDVSGLLNSGEIKLGLSHDISHVQEIGGTTITVKRRASFFSRKKGKLIADVIEGDRVYLENTEAAVVRGKEVIIG-PGCSIGTIEYEYKCECDPHSQIKEKTKL- 2R5R Chain:A ((23-268)) HIAIDRVGIKAIRHPVVVADKGGGSQHTVAQFNMYVNLPHNFKGTHMSRFVEILNSHEREISVESFEEILRSMVSRLESDSGHIEMAFPYFINKSAPVSGVKSLLDYEVTFIGEIKHGNQYSFTMKVIVPVTSLCPCSKKISDYG----AHNQRSHVTISVRTNSFIWIEDIIRIAEEQA--SCELYGLLKRPDEKYVTERAYNNPKFVEDIVRDVAEVLNHDDRIDAYIVESENFESIHNHSAYALIERDK

General information: TITO was launched using: RESULT: Template: 2R5R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1057 -39503 -37.37 -172.50 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -37.37 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.117

(partial model without unconserved sides chains): PDB file : Tito_2R5R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R5R-query.scw PDB file : Tito_Scwrl_2R5R.pdb: